Liqui-CultTM testing determines the type and degree of infestation. . To use, simply fill the 5cc syringe (included in your kit) with the fluid to be tested. Inject the complete 5cc's through the rubber cap of the test bottle containing the liquid agar and shake it vigorously. Incubate for 30 hours for bacterial growth or 72 hours for fungal growth and compare it to the appropriate photo charts to determine the degree of contamination.